Urease from Canavalia ensiformis (Jack bean), Type III, powder, 15,000-50,000 units/g solid

Urease from Canavalia ensiformis (Jack bean), Type III, powder, 15,000-50,000 units/g solid

Stock Code: 3581502
Manufacturer Part No: U1500-1MU
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Biochem/physiol Actions


Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.


General description


Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620


Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.


Packaging


20000, 100000, 500000, 1000000 units in poly bottle


Unit Definition


One micromolar unit will liberate 1.0 µmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Quality Level300
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formpowder

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