Trypsin Acetylated from bovine pancreas, Type V-S, >=8,500 BAEE units/mg protein (biuret)

Trypsin Acetylated from bovine pancreas, Type V-S, >=8,500 BAEE units/mg protein (biuret)

Stock Code: 3581444
Manufacturer Part No: T6763-100MG
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£160.18 (exc VAT) per 100MG
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Application


Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.


Biochem/physiol Actions


Lys-acetylated trypsin is less susceptible to autolysis than the native form.


General description


The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.


Other Notes


View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.


Packaging


1 g in glass bottle


100, 250 mg in glass bottle


Preparation Note


Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).


Unit Definition


One BAEE unit will produce a δA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formpowder

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