Thrombin

Stock Code: 3581469
Manufacturer Part No: T7572-1000UN
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Analysis Note


The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.


Application


Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an innovative flow cytometric approach for small-size platelet microparticles.


Biochem/physiol Actions


Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.


General description


Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.


Lyophilized powder containing sucrose, sodium chloride and Tris.


Other Notes


View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.


Unit Definition


Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
ImpuritiesHIV, hepatitis B and hepatitis C, tested negative
Formlyophilized powder

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