Thrombin from bovine plasma, lyophilized powder, >=2,000 NIH units/mg protein (E1%/280 = 19.5)

Thrombin from bovine plasma, lyophilized powder, >=2,000 NIH units/mg protein (E1%/280 = 19.5)

Stock Code: 3581468
Manufacturer Part No: T7513-50UN
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Analysis Note


The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.


Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.


Biochem/physiol Actions


Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.


Other Notes


View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.


Physical form


Lyophilized from saline sodium citrate buffer, pH 6.5


Unit Definition


Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

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