SSC Buffer, powder blend

Stock Code: 3577450
Manufacturer Part No: S0902-1L
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£121.79 (exc VAT) per 1L
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Application


Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.


Suitable for: Preparing nucleic acids for hybridization Hybridization buffer for Northern and Southern blots Washing buffer for Northern and Southern blots


Components


Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.


Features and Benefits


Multi-purpose use in hybridizations Easily reconstituted and diluted to any concentration needed


General description


SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Quality Level200
Pack1L
pH6.8-7.2 (20-25 °C, 263 mg/L in water)
ManufacturerSIGMA-ALDRICH
Storage Temp.room temp
Solubilitywater: 263 mg/mL, clear, colorless
Formpowder blend

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