S-Gal, reagent for selection of recombinant bacterial clones

S-Gal, reagent for selection of recombinant bacterial clones

Stock Code: 3577233
Manufacturer Part No: S9811-100MG
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£124.38 (exc VAT) per 100MG
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Application


S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.


Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving.


Features and Benefits


More intense color contrast than X-gal Excellent for use in automated colony counters Autoclavable for easiest use No need to make stock solutions


When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.


General description


S-Gal® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.


Legal Information


S-GAL is a registered trademark of Sigma-Aldrich Co. LLC


Other Notes


The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.


Autoclavable, microwavable growth medium complete with S-Gal and IPTG.


Principle


When S-Gal® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.


Reconstitution


Stock solutions of S-Gal® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.

Quality Level100
InChI keyHDJJDRXZHJRVGA-DIKXUDHVSA-N
InChI1S/C19H22O9/c20-7-14-15(22)16(23)17(24)19(28-14)27-13-6-12-10(5-11(13)21)8-3-1-2-4-9(8)18(25)26-12/h5-6,14-17,19-24H,1-4,7H2/t14-,15+,16+,17-,19-/m1/s1
ManufacturerSIGMA-ALDRICH
SolubilityDMF: 50 mg/mL
Formpowder
Assay≥95% (HPLC)

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