ROCHE X-Gal5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside

ROCHE X-Gal5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside

Stock Code: 2795457
Manufacturer Part No: 10745740001
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Application


X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.


X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.


General description


X-Gal is a chromogenic substrate for ?-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Physical form


Crystals, white powder


Preparation Note


Working solution: Stock solution: 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution A590 nm: 0.01Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.


Quality


Chromatographically homogeneous

Quality Level100
InChI keyOPIFSICVWOWJMJ-AEOCFKNESA-N
InChI1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
Further Info5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
Solubilitymethanol: soluble, DMF: soluble

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