ROCHE T4 DNA Ligaserecombinant form of the enzyme from T4 phage

ROCHE T4 DNA Ligaserecombinant form of the enzyme from T4 phage

Stock Code: 2795426
Manufacturer Part No: 10481220001
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Application


Ligation of sticky- and blunt-ended DNA fragments.


General description


T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.ContentsT4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Preparation Note


Activator: Mg2+ (10mM), DTTWorking solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).


Quality


QualityTested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.


Specificity


Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).


Unit Definition


One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol 32P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 µg/ml, pH 7.6 (+25 °C).Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml

Quality Level100
Parameter4-22 °C optimum reaction temp.
Further Inforecombinant form of the enzyme from T4 phage
Formsolution

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