ROCHE Proteinase K Recombinant PCR Grade Solution, 1.25mL

Stock Code: 2795218
Manufacturer Part No: 3115887001

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Descriptions

Application

This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.The enzyme promotes cell lysis by activating a bacterial autolytic factor.Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.The enzyme is particularly well suited for isolating nucleic acids for amplification reactions.Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

Features and Benefits

Proteinase K cleaves proteins between amino acids X and Y (X-_↓-Y), when X = an aliphatic, aromatic, or hydrophobic amino acid, and Y = any amino acid. The enzyme is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. Choose an effective tool for template preparation. Inactivate DNases and RNases of most species. Count on consistent quality and performance. Stringent quality testing ensures optimal stability and high-level lot-to-lot performance. Prepare samples over a wide range of conditions. The robust enzyme is stable over a wide pH range and is ideal for diverse applications. Benefit from a contamination-free enzyme. The enzyme is tested for the absence of RNases and DNases, and is virtually free of DNA. It is especially suited for the isolation of PCR templates.

General description

Proteinase K is a subtilisin-related serine protease that has no pronounced cleavage specificity.The enzyme is also available as a lyophilizate.

The recombinant enzyme is identical to the native protease originally isolated from the mold Tritirachium album. The specifications of the recombinant enzyme are the same as those of the native protease. The amino acid sequence (molecular weight) and the molecule structure are identical. However, the recombinant preparation is much purer than the native enzyme, as it is DNA-free, according to the current quality control procedures, and is therefore well suited for isolating PCR and RT-PCR templates.

Legal Information

Pefabloc is a registered trademark of Pentapharm

Other Notes

For life science research only. Not for use in diagnostic procedures.

For the rapid inactivation of RNases and DNases.The enzyme can reduce protein to free amino acids if it is present in large excess for long incubation periods.

Preparation Note

Activator: To stimulate proteinase K activity, denaturing agents (SDS and urea) can be added. For example, SDS at a final concentration of 2% can increase the activity of proteinase K significantly. Optimization using denaturing agents can increase proteinase activity by as much as sevenfold.Working solution: Suggested Buffers:The most appropriate buffer for Proteinase K will vary from application to application. Always follow the pH and temperature guidelines in parameter filed