ROCHE Protein A Agarose

ROCHE Protein A Agarose

Stock Code: 2795541
Manufacturer Part No: 11134515001
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Application


Purification of mouse monoclonal antibodies from ascites fluid or cell culture supernatants. Protein A or Protein G Agarose affinity matrix can be used to purify antibodies from crude cell extracts and for the immunoprecipitation or co-immunoprecipitation of proteins. It has also been used for photoactivatable-ribonucleoside-enhanced cross-linking immunoprecipitation (PAR-CLIP) analysis.


Components


Preswollen gel, ready-to-use, 2 ml or 5 ml gel (settled gel volume) in buffer that contains 20% ethanol as a preservative, nonsterile.Protein A content: 3 mg/ml pre-swollen gel


General description


The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times, depending on the elution condition.Protein A leakage: ﹤18 ng Protein A/ml (ELISA)Regeneration: the gel can be used approximately 30 timesStructure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gelProtein A, immobilized.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Quality


Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.


Specificity


Protein A is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein A binds to varying degrees IgM, IgA, IgD, and most subclasses of IgG.

Capacity>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)
Storage Temp.2-8°C
Formsuspension
Assay>98% (HPLC and SDS-PAGE)

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