ROCHE Liberase DH Research Grade 10 Mg

Stock Code: 2795318
Manufacturer Part No: 5401054001

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Descriptions

Application

Liberase^™ DH (Dispase High) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.It has been used for the digestion of metastatic tumor fragments. It has been used for the enzymatic detachment of human embryonic stem cells colonies.

Features and Benefits

Maximize viability and yield of isolated cells with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content. Count on higher specific activity of the enzyme blend as a result of higher Collagenase I + II purity (determined by HPLC analysis). Obtain higher experimental reproducibility due to higher lot-to-lot consistency.

General description

The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.Liberase enzyme technology comprises methods for purifying clostridial collagenase isoforms to high specific activity, and for blending them together with high-specific-activity neutral protease in optimal ratios for effective dissociation of primary tissues and cultured cells. Liberase DH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a high concentration of Dispase^®, a non-clostridial neutral protease.Liberase enzymes are designed to increase the quality and reproducibility of tissue dissociation, and improve the viability and functionality of isolated cells. Liberase Purified Enzyme Blends replace traditional collagenase, which is a crude and variable fermentation by-product of Clostridium histolyticum.

Legal Information

Liberase is a trademark of Roche

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Other Notes

This product is intended for life science research and in vitro use only. These products are not to be used for diagnostic or clinical applications, such as human islet transplantation. All 1st generation Liberase and Liberase Blendzyme products (Liberase HI, CI, RI, PI, Liberase Blendzyme 1, 2, 3, 4) have been replaced by the 2nd generation Liberase Research Grade and Liberase GMP grade enzyme blend portfolios. For instructions concerning the transition from previously used Liberase EnzymesFor life science research only. Not for use in diagnostic procedures.

Preparation Note

Stabilizers: CalciumWorking concentration: Liberase Research Grade Enzyme Working ConcentrationLiberase enzymes have significantly higher specific activities than traditional collagenases. This means that the working concentration of Liberase Research Grade Purified Enzymes, expressed in mg/ml, will be lower than that of traditional collagenase.When the application is on the Roche list of applications at www