ROCHE Faststart Taq DNA Polymerase 1000U

Stock Code: 2795761
Manufacturer Part No: 12032937001

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Descriptions

Application

FastStart^™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step. It can be applied for:PCRMultiplex PCRDifficult templates e.g., secondary structures or GC-rich sequencesAutomated PCR e.g., handling at room temperaturesHot Start PCR up to 3kbHot Start RT-PCR up to 3kbQuantitative reverse transcription PCR (RT-qPCR)Bisulfite-specific PCRUse FastStart^™ Taq DNA Polymerase, dNTPack with ready-to-use PCR nucleotide mix.For maximum convenience, select the 2× concentrated ready-to-use FastStart^™ PCR Master.

Features and Benefits

FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive at temperatures below +75°C. The kit includes an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates. High enzyme stability enables pipetting by robotic stations. Higher specificity, sensitivity, and yield: Hot start PCR makes PCR setup easier. Use robotic setup.Use this enyzme mix stable for 24hours at +15 to +25°C. Prevent PCR carryover contamination.Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes optimized polymerase chain reaction (PCR) buffer system and a GC-RICH solution for handling wide range of templates superior results due to its unique enzyme design and optimized buffer system

General description

FastStart^™ Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart^™ Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. FastStart^™ Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.

Legal Information

FastStart is a trademark of Roche

NOTICE TO PURCHASER: LIMITED LICENSE﹤br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only