ROCHE Faststart High Fidelity PCR System 2500u

Stock Code: 2795242
Manufacturer Part No: 3553361001

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Descriptions

Application

The FastStart^™ High Fidelity PCR System — a new enzyme blend for hot start PCR — combines all the benefits of FastStart^™ Taq DNA Polymerase with fourfold higher accuracy and the abilty to amplify fragments up to 5 kb. PCRMultiplex PCRRT-PCRGC-rich template amplificationDifficult or challenging PCRsin the amplification of Human Papillomaviral genome and methylated DNAThe FastStart^™ High Fidelity PCR System is the product of choice for multiplex PCR.Use FastStart^™ High Fidelity PCR System, dNTPack with ready-to-use PCR Nucleotide Mix.

Features and Benefits

Achieve excellent multiplex performance. For fast high quality results, also use Roche′s PCR Optimization Kit. Increase fidelity. Achieve fourfold higher fidelity compared to Taq DNA Polymerase and FastStart Taq DNA Polymerase. Amplify challenging DNA. DMSO, a PCR additive, for working with difficult templates, is supplied.

General description

The FastStart^™ High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75 °C and are activated by heating to +95 °C for two minutes.Hot start protocols have improved specificity, sensitivity, and PCR yield. Heat-labile blocking groups on amino acid residues of FastStart^™ Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically. FastStart^™ Taq DNA Polymerase is activated by removal of blocking groups using a preincubation step at +95°C. The FastStart^™ High Fidelity PCR System is the product of choice for multiplex PCR. FastStart^™ High Fidelity Enzyme Blend, (5 U/µl) in storage buffer FastStart^™ High Fidelity Reaction Buffer, 10x concentrated, with 18 mM MgCl₂ FastStart^™ High Fidelity Reaction Buffer, 10x concentrated, without MgCl₂ MgCl₂ Stock Solution, 25 mM DMSO

Legal Information

FastStart is a trademark of Roche

NOTICE TO PURCHASER: LIMITED LICENSE﹤br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific