ROCHE Beta-glucuronidase From E.coli K12 Solution In 50

Stock Code: 2795245
Manufacturer Part No: 3707580001

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Descriptions

Application

?-Glucuronidases have been used extensively in research and analytical laboratories for the enzymatic hydrolysis of steroid ?-glucuronides. ?-Glucuronidase is used:for the hydrolysis of steroid conjugates (glucuronides) in urine (pH 6.0–6.5)in doping analysisfor the detection of benzodiazepine in small doses.during sample preparation to cleave off glucuronides prior to GC-MS, HPLC, immunoassays, or other analytical methods.It has been used for the hydrolysis of plasma enterolactone, testosterone and epitestosterone glucuronide.

Biochem/physiol Actions

Glucuronidation is one of the basic principles of metabolism. Many substances presented to the human body undergo metabolic processing that includes conjugation with glucuronic acid by UDP-glucuronosyltransferases (UGTs). ?-Glucuronidase, also known as ?-D-glucuronoside glucuronosohydrolase, catalyzes the transfer of a glucuronyl group to many biological and pharmacologically active endogenous and exogenous molecules. Glucuronide is in general, more soluble, less toxic, and more easily excreted by the human body compared to the original molecule. To analyze these drug conjugates that are present in body fluids, such as urine and plasma, deconjugation of the glucuronide is necessary. Enzymatic hydrolysis prior to detection is essential to achieving high sensitivity during analysis. After hydrolysis, the sample may be analyzed by mass spectroscopy, gas chromatography, HPLC, immunoassays, or other analytical methods.

Features and Benefits

?-Glucuronidase from E. coli is highly specific for ?-glucuronides, and is capable of very quickly hydrolyzing steroid ?-glucuronides as well as cleaving many other types of glucuronides, such as benzodiazepine, opioid, and cannabinoid. ?-Glucuronidase is used for the enzymatic hydrolysis of glucuronides in biological fluids, primarily urine. Substances used for doping are conjugated with glucuronides, which means that effective doping analysis relies very often on the enzymatic cleavage of conjugated drug molecules by ?-glucuronidase. Perform fast analysis due to the enzyme?s high specific activity.Quickly screen for steroids, benzodiazepines, cannabinoids, and opioids.Save time by developing your procedure without the need to clean up the reaction or buffer the urine.

General description

β-D-Glucuronoside glucuronosohydrolase

Glucuronidation is one of the basic principles of metabolism. Many substances presented to the human body undergo metabolic processing that includes conjugation with glucuronic acid by UDP-glucuronosyltransferases (UGTs). This enzyme catalyzes the transfer of a glucuronyl group to many biological and pharmacologically active endogenous and exogenous molecules. Glucuronide is in general, more soluble, less toxic, and more easily excreted by the human body compared to the original molecule. To analyze these drug conjugates that are present in body fluids, such as urine and plasma, deconjugation of the glucuronide is necessary.Substances used for doping are conjugated with glucuronides, which means that effective doping analysis relies very often on the enzymatic cleavage of conjugated drug molecules by β-glucuronidase.Enzyme CharacteristicsThe E