ROCHE Acetyl-Coenzyme A

Stock Code: 2795345
Manufacturer Part No: 10101893001

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Descriptions

Application

Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts. Acetyl-Coenzyme A has also been used to determine citrate synthase activity.

Biochem/physiol Actions

Acetyl-Coenzyme A (Ac-CoA) is the end product of glycolysis and takes part in the Ac-CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. It also takes part in fatty acid biosynthesis and catabolism of polyamines like spermine and spermidine. A low level of Ac-CoA leads to a loss in glial cell and neuronal function. Ketone bodies and triglycerides give rise to Ac-CoA on hydrolysis and this indirectly leads to increased histone acetylation.

General description

Acetyl-Coenzyme A, trilithium salt

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).Storage conditions (working solution): -15 to -25 °C50 mg/ml in phosphate buffer, pH 7, is stable for 3 weeks at -15 to -25 °C. Unfrozen solutions should be immediately used.