Pyruvate Kinase from rabbit muscle, Type II, ammonium sulfate suspension, 350-600 units/mg protein

Pyruvate Kinase from rabbit muscle, Type II, ammonium sulfate suspension, 350-600 units/mg protein

Stock Code: 3579982
Manufacturer Part No: P1506-25KU
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Analysis Note


Protein determined by biuret.


Application


Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of ?-substituted carboxylic acids.


Biochem/physiol Actions


Molecular Weight: 237 kDa?and exists as a tetramer of four equal subunits of molecular weight 57 kDa.Isoelectric Point: 7.6Optimal pH: ~7.5 Optimal Temperature: 25°C εA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.


Pyruvate kinase from rabbit muscle can be activated by histidine and inhibited by low levels of zinc (Zn2+).


General description


Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.


Physical form


Suspension in 3.2 M (NH4)2SO4 solution, pH 6


Unit Definition


One unit will convert 1.0 µmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37?°C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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