Pyronin Y, RNA staining tested

Stock Code: 3582113
Manufacturer Part No: P9172-1G

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Descriptions

Application

Pyronin Y has been used: in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)staining of CD34⁺ cells

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Packaging

1, 5 g in glass bottle

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Specifications

Quality Level	200
mp	250-260 °C (lit.)
InChI key	INCIMLINXXICKS-UHFFFAOYSA-M
InChI	1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
Manufacturer	SIGMA-ALDRICH
Composition	Dye content, ≥45%
Form	powder