Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-

Stock Code: 3581054
Manufacturer Part No: P3910-500MG
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£250.71 (exc VAT) per 500MG
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Application


This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).


Biochem/physiol Actions


Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .


Packaging


500 mg in poly bottle


Physical properties


Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.


Specificity


Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.


Unit Definition


One unit will hydrolyze casein to produce color equivalent to 1.0 µmole (181 µg) of tyrosine per minute at pH 7.5 at 37°C.

Quality Level100
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formlyophilized powder

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