Pectinase from Aspergillus niger, BioReagent, suitable for plant cell culture, aqueous glycerol solu

Stock Code: 3581084
Manufacturer Part No: P4716-100KU

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Descriptions

Application

Petctinase from Aspergillus niger has been used: for protoplasts liberation in the protoplast enzyme mixture for enzyme digestion to conduct partial saccharification of sugarsto study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Biochem/physiol Actions

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.

General description

We are committed to bringing you?Greener?Alternative Products, which adhere to one or more of The 12 Principles of?Greener?Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

25000, 100000 units in glass bottle

Physical form

Solution in 40% glycerol

Unit Definition

One unit will liberate 1.0 µmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25?°C.