TRANSCRIPTOR ONE-STEP RT-PCR KIT 150 RX

Stock Code: 2795261
Manufacturer Part No: 4655885001

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Descriptions

Application

The Transcriptor One-Step RT-PCR Kit is designed for fast, sensitive, and specific end-point RT-PCR analysis of RNA (total RNA, mRNA, in vitro-transcribed RNA, or viral RNA) using gene-specific primers.Featuring an innovative reaction buffer, the kit combines sensitive, high-yield reverse transcription with the improved performance of a hot start system for high-fidelity, high-yield amplification of a variety of templates, including GC-rich RNA.The kit offers a convenient, highly sensitive one-step RT-PCR method to generate transcripts up to 6.5 kb for applications such as: Qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels Determining the presence or absence of RNA templates Quantifying gene expression through RNA analysis (e.g., for virus-level quantification) Cloning of RNA up to 6.5 kbThe one-step format is convenient, reduces reaction-to-reaction variability, and prevents contamination by minimizing hands-on steps.

Transcriptor One-Step RT-PCR Kit has been used in the amplification of:hepatitis C virus (HCV) fragmentsheavy chain CH3 region and light chain polyA regionmurine leukemia virus (MLV)

Components

RT-PCR Enzyme Mix containing Transcriptor Reverse Transcriptase, Protector RNase Inhibitor, and the Expand System RT-PCR Reaction Buffer containing hot start mediating components and dNTPs – 5x concentrated Water, PCR Grade Control RNA – HAV RNA, in vitro transcript * Control primer mix HAV RNA (forward and reverse primer) ** Included only in the 50-reaction pack size.

Features and Benefits

Obtain high sensitivity and yield in a wide amplification range:Detect as little as 1 fg of total RNA. Achieve high specificity and reduce primer-dimersCount on excellent performance with the kit’;s proprietary hot start buffer (patents pending). Produce long fragments:Generate transcripts up to 6.5 kb. Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA):Overcome high secondary structure with thermostableTranscriptor Reverse Transcriptase in combination with an optimized buffer. Save time and improve results with a convenient kit:Minimize pipetting steps and prevent contamination by using a premixed enzyme blend and optimized buffer that includes PCR-grade dNTPs and hot start components. Protect your RNA sample:Safeguard your RNA from degradation by RNases with the supplied thermostable Protector RNase Inhibitor that is functional during one-step RT-PCR when other RNase inhibitors fail. Increase confidence in your results:Use the supplied control reactions to monitor performance.

General description

One-step RT-PCR is a very sensitive technique for determining the presence or absence of RNA templates, or to quantify the levels of expression through qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels (e.g., for virus-level quantification). This RT-PCR method also facilitates the amplification of rare messages for cloning. The one-step format is convenient, shows reduced reaction-to-reaction variability, and prevents contamination by minimizing hands-on steps