o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet

Stock Code: 3578662
Manufacturer Part No: P3804-100TAB

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Descriptions

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Packaging

16 mg total tablet weight

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 µl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Specifications

Quality Level	200
mp	258 °C (dec.) (lit.)
InChI key	RIIWUGSYXOBDMC-UHFFFAOYSA-N
InChI	1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
Manufacturer	SIGMA-ALDRICH
Storage Temp.	2-8°C
Solubility	water: 1 tablet/10 mL, clear, colorless
Form	tablet