Nitrilase, recombinant, expressed in E. coli, >=2.0 U/mg

Stock Code: 3578815
Manufacturer Part No: 04529-10MG
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£126.33 (exc VAT) per 10MG
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Application


Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.


Biochem/physiol Actions


Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.


General description


Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).


Packaging


Bottomless glass bottle. Contents are inside inserted fused cone.


Unit Definition


1 U corresponds to the amount of enzyme which liberates 1 µmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid

Quality Level100
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formcrystals, powder

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