MTT Formazan, MTT reduction end product

MTT Formazan, MTT reduction end product

Stock Code: 3578019
Manufacturer Part No: 88417-1G
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£181.07 (exc VAT) per 1G
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Application


MTT Formazan has been used in cell viability assay.


Biochem/physiol Actions


MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.


Other Notes


Reviews: Tetrazolium salts and formazans

Quality Level100
InChI keyGIFKVQOPAJDJLO-SZVLODPRSA-N
InChI1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
ManufacturerSIGMA-ALDRICH
Solubilitychloroform: 10 mg/mL, clear, dark violet
Formpowder

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