Monoclonal ANTI-FLAG M5 antibody produced in mouse, clone M5, purified immunoglobulin, buffered aque

Stock Code: 3586954
Manufacturer Part No: F4042-1MG

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Descriptions

Application

Monoclonal ANTI-FLAG^® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.

Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca²⁺-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

General description

Monoclonal ANTI-FLAG^® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Antibody is not calcium dependent.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Preparation Note

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.