Monoclonal ANTI-FLAG M1 antibody produced in mouse, clone M1, purified immunoglobulin, buffered aque

Monoclonal ANTI-FLAG M1 antibody produced in mouse, clone M1, purified immunoglobulin, buffered aque

Stock Code: 3586951
Manufacturer Part No: F3040-1MG
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£1,104.54 (exc VAT) per 1MG
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Application


Monoclonal ANTI-FLAG® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Chromatin immunoprecipitation (1 paper)Immunofluorescence (1 paper)Browse additional application references in our FLAG® Literature portal.


Biochem/physiol Actions


Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.


General description


The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.Method of purification - Protein A


Immunogen


FLAG; peptide sequence DYKDDDDK


Legal Information


FLAG is a registered trademark of Sigma-Aldrich Co. LLC


ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC


Other Notes


M1 antibody binding is calcium dependent.


Physical form


Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).


Preparation Note


Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

ManufacturerSIGMA-ALDRICH
Formbuffered aqueous solution

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