Lipase from porcine pancreas, Type II, 100-400 units/mg protein (using olive oil (30 min incubation)

Stock Code: 3579795
Manufacturer Part No: L3126-500G
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£324.54 (exc VAT) per 500G
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Analysis Note


Protein determined by biuret.


Application


Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.


Lipase from porcine pancreas has been used:for the analysis of fatty acid at the 2-position of triglycerides.to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.as a component of dissolution media to study the dissolution profiles of theophylline.


Biochem/physiol Actions


Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.


Packaging


25, 100, 500 g in poly bottle


Quality


Crude (Steapsin). Contains amylase and protease activity.


Unit Definition


One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Quality Level200
ManufacturerSIGMA-ALDRICH
Compositionprotein, ≥20%
Storage Temp.2-8°C
Formlyophilized (cake)

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