KAPA TAQ HS+DNTPS (250U)

Stock Code: 2795921
Manufacturer Part No: KK1509

Order Now for 7 day delivery

Descriptions

Application

KAPA Taq HotStart^® has been used for:High throughput PCRAmplification of low copy DNA templatesMultiplex PCRSpecific amplification of complex templatesRT-PCRAquarium DNA extraction to generate marker gene libraries

Biochem/physiol Actions

KAPA Taq HotStart^® DNA polymerase and KAPA Taq exhibit 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The enzyme shows an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotstart buffer is formulated uniquely to facilitate specific primer annealing. This translates to higher yields of the specific product when compared to traditional Taq buffers. It also enhances the amplification of GC- and AT-rich templates. KAPA Taq hotstart DNA polymerase might be used in combination with any standard Taq buffer with a pH of 8.3 or higher

Features and Benefits

High performance Improved sensitivity, specificity, and yields. Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.Quick Notes: KAPA Taq HotStart^® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl₂ concentration and annealing temperature may need to be optimized to account for differences in formulation. The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates. The KAPA Taq HotStart Buffer does not contain MgCl₂; MgCl₂ (25 mM) is supplied separately to allow greater flexibility during reaction setup. The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

General description

KAPA Taq DNA Polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli. KAPA Taq DNA Polymerase has 5'g3' polymerase and 5'g3' exonuclease activity, but no 3'g5' exonuclease (proofreading) activity. The enzyme system has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This prevents nonspecific amplification during reaction setup, increases sensitivity, and improves reaction efficiency. PCR products generated with KAPA Taq HotStart are A-tailed and may be cloned into TA cloning vectors. KAPA Taq HotStart Buffer is a uniquely formulated buffer to facilitate specific primer annealing. This translates to higher yields of specific product when compared to traditional Taq buffers, and improved amplification of GC- and AT-rich templates. However, KAPA Taq HotStart DNA Polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.