IMAC-Select Affinity Gel,

IMAC-Select Affinity Gel,

Stock Code: 3587007
Manufacturer Part No: I1408-5ML
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£162.96 (exc VAT) per 5ML
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Application


An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.


Other Notes


Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.


Physical form


1:1 suspension in a 30% ethanol solution


Preparation Note


The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Capacity10-30 µmol/mL binding capacity (Cu+2)
Quality Level200
ManufacturerSIGMA-ALDRICH
Matrix6% beaded agarose
Storage Temp.2-8°C
Impurities≤0.08 Metal Contamination (µmol/ml packed gel)
Form(1:1 suspension in a 30% ethanol solution)

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