GenElute#-E Cell Culture DNA Kit

GenElute#-E Cell Culture DNA Kit

Stock Code: 3586859
Manufacturer Part No: EC400-10RXN
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Application


The GenElute™-E Single Spin Cell Culture DNA Kit purifies genomic DNA from common cultured cell lines. DNA from up to 2 x 10^6 fresh or frozen cells can purified using a standard protocol, without the need for additional optimizations.The initial lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This eliminates the requirement for extended, overnight incubations, which are required in many conventional protocols.Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is equal or better than silica-based methods depending on sample type.?GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.


Features and Benefits


Fast and efficient purification: 10-minute lysis and minimal hands-on, preparation stepsHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals


General description


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GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers: Isolation of DNA in under an hour with minimal sample handling Better purity, leading to improved performance in PCR and other applications Significant reduction in plastic and hazardous chemical wasteTraditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.GenElute™-E kits employ size exclusion?negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and?plastic?materials required for purification.?This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.


ManufacturerSIGMA-ALDRICH
FeatureCompatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please

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