Fluorescein di(beta-D-galactopyranoside),

Stock Code: 3586944
Manufacturer Part No: F2756-5MG

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Descriptions

Application

Fluorescein di(?-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).

Fluorescein di(?-D-galactopyranoside) has been used as a substrate in ?-galactosidase assay.

Biochem/physiol Actions

Fluorescein di (?-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by ?-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using ?-galactosidase (Lac Z) as a reporter gene.

Packaging

1, 5, 25 mg in glass insert

Substrates

Fluorogenic substrate for β-galactosidase

Specifications

Quality Level	100
InChI key	ZTOBILYWTYHOJB-WBCGDKOGSA-N
InChI	1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(4
Manufacturer	SIGMA-ALDRICH
Storage Temp.	−20°C
Solubility	methanol: 20 mg/mL, clear, yellow
Form	powder
Assay	≥98% (HPLC)