Faststart Taqman Probe Master 200x50ul

Stock Code: 2795262
Manufacturer Part No: 4673409001

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Descriptions

Application

The FastStart TaqMan^® Probe Master has been used in:quantitative, real-time DNA-detection assaysqPCR and two-step reverse transcription (RT)-qPCRin the hydrolysis probe detection format

Components

FastStart TaqMan^® Probe Master, 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, and Nucleotides (dATP, dCTP, dGTP, dUTP).

Features and Benefits

Increase qPCR sensitivity and specificity:Produce lower cycle threshold (C_t) values with the hot start enzyme FastStart Taq DNA Polymerase and highest purity nucleotides included in the master mix. Use it with any probe-based assay:Achieve sensitive, specific results in assays with the Universal ProbeLibrary Probes or any other hydrolysis probe. Amplify and detect a broad range of DNA or cDNA targets: Amplify fragments up to 500 bp long, including those that are GC- or AT-rich. Use with any real-time qPCR instrument other than the LightCycler^® Instruments:Choose from two formulations - one that contains the ROX reference dye and one without ROX. Visualize amplification products on agarose gels. Use robotic pipetting stations to set up qPCR reactions:Benefit from a master mix that is stable at room temperature during extended reaction setup times. Prevent carryover contamination:The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).

General description

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. The FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).

FastStart TaqMan^® Probe Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycle^® instruments. It is a 2x concentrated master mix that contains all the reagents (except primers, probe, and template). This master mix simplifies the preparation of reactions for DNA detection and analysis. It allows very sensitive detection and quantification of defined DNA sequences.Hot start protocols have been also been shown to significantly improve the specificity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can non-specifically bind. The FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).This FastStart TaqMan^® Probe Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich