FASTSTART SYBR GREEN MASTER 50 ML

Stock Code: 2795265
Manufacturer Part No: 4673492001

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Descriptions

Application

The FastStart^™ SYBR^® Green Master has been used in qPCR and two-step qRT-PC in the SYBR^® Green I detection format. It is also used: in quantitative polymerase chain reaction (qPCR) for adeno-associated virus (AAV) titre quantificationin qPCR to determine the expression level of different Col6a1-3 genes relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH)in quantitative real-time polymerase chain reaction (qRT-PCR) of G protein-coupled estrogen receptor-1 (GPER) mRNAin qRT-PCR for gene expression studiesUse the FastStart^™ SYBR^® Green Master with any real-time qPCR instrument other than the LightCycler^® Instruments.

Components

FastStart SYBR Green Master, 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and SYBR Green I.

Features and Benefits

Improve PCR sensitivity and specificity:Rely on this mix′s FastStart Taq DNA Polymerase to minimize the formation of nonspecific amplification products through hot start PCR. Avoid over-estimation of qPCR results:Eliminate nonspecific amplification products and primer-dimers that would increase the amount of bound quantified SYBR Green I. Amplify and detect a broad range of DNA or cDNA targets:Amplify fragments up to 500 bp long, including those that are GC- or AT-rich. Works with any real-time PCR instrument other than the LightCycler^® Instruments. Use any real-time PCR instrument other than the LightCycler^® Instruments:Choose from two formulations - one that contains the ROX reference dye and one without ROX. Save time and effort in qPCR preparation:Rely on this easy-to-use 2x master mix to eliminate the need to mix components, titrate MgCl2, or perform other time-consuming optimization steps. Prevent false positives resulting from carryover contamination:The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.

General description

FastStart SYBR Green Master; Instructions For UseFastStart^™ SYBR^® Green Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycler^® instruments. This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart^™ TaqMan^® Probe Master allows very sensitive detection and quantification of defined DNA sequences.SYBR^® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR^® Green I dye, included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart^™