Ethylene glycol-bis(beta-aminoethyl ether)-N,N,N,N-tetraacetic acid tetrasodium salt, >=97%

Stock Code: 3586230
Manufacturer Part No: E8145-10G

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Descriptions

Application

A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca²⁺.

EGTA is suitable for use:in media for chelating Ca²⁺ to facilitate efficient incorporation of Sr²⁺ into the oocytesas a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteinsas a component of oocyte activation media

Biochem/physiol Actions

Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca²⁺ in the presence of Mg²⁺ at a ratio of 1:1.

Ethylene glycol-bis(?-aminoethyl ether)-N,N,N?,N?-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).

Packaging

10, 50 g in glass bottle

Specifications

Quality Level	200
InChI key	BAOGCDPNNOBWQZ-UHFFFAOYSA-J
InChI	1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4
Manufacturer	SIGMA-ALDRICH
Solubility	H2O: soluble 10%, clear, colorless
Assay	≥97%