DFHBI is a membrane permeable and nontoxic fluorogen, which produces fluorescence upon binding. RNA aptamers Spinach, Spinach2 and Broccoli. DFHBI displays fluorescence excitation maxima of 447 nm and peak fluorescence emission of 501 nm. DFHBI is used for imaging RNA in cells.
3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) is non fluorescent when unbound.