Citrate Synthase from porcine heart, ammonium sulfate suspension, >=100 units/mg protein

Citrate Synthase from porcine heart, ammonium sulfate suspension, >=100 units/mg protein

Stock Code: 3579162
Manufacturer Part No: C3260-1KU
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Application


Citrate Synthase from porcine heart has been used:to inject newt egg for determining its importance in egg activation at fertilizationin the preparation of reaction mix to determine pyruvate carboxylase enzyme activityto examine whether it can induce a [Ca2+]increase and egg activation in unfertilized eggs


Biochem/physiol Actions


Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.


Physical form


Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.


Preparation Note


Dissolves in water to form a clear solution at 1 mg/mL concentration.


Unit Definition


One unit will form 1.0 µmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
SolubilityH2O: soluble 1.0 mg/mL, clear
Formammonium sulfate suspension

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