beta-Glucuronidase from limpets (Patella vulgata), Type L-II, lyophilized powder, 1,000,000-3,000,00

Stock Code: 3579635
Manufacturer Part No: G8132-250KU

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Descriptions

Application

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

?-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine.

Biochem/physiol Actions

?-glucuronidase (?-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-?-D glucuronide was converted to morphine using ß-glucuronidase obtained from Patella vulgate.

Packaging

100000, 250000, 500000, 1000000, 2000000 units in poly bottle

Quality

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).