beta-Galactosidase from Escherichia coli, Grade VI, lyophilized powder, 250-600 units/mg protein

Stock Code: 3579573
Manufacturer Part No: G6008-10KU
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Application


?-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). ?-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.


Biochem/physiol Actions


?-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of ?-galactosidase, in a feedback loop.


β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.


General description


?-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.


Packaging


10000 units in poly bottle


1000, 5000 units in glass bottle


Physical form


Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol


Physical properties


Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)


Unit Definition


One unit will hydrolyze 1.0 µmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥50% biuret
Formlyophilized powder

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