beta-Amylase from sweet potato, Type I-B, ammonium sulfate suspension, >=750 units/mg protein (E1%/2

Stock Code: 3579018
Manufacturer Part No: A7005-10KU

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Descriptions

Application

? -Amylase is used to hydrolyze ? bonds of ?-linked polysaccharides, such as starch and glycogen. ? -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. ?-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms. The enzyme has also been used to prepare ?-limit dextrin from waxy maize starch.

?-Amylase from sweet potato has been used:in the hydrolysis of starch and starch-pectin combinationsfor comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assayfor selenium speciation in samples

Biochem/physiol Actions

?-Amylases plays a major role in examining enzymatic promiscuity among glycosidases.

?-Amylase hydrolyzes the ?-(1,4) glucan linkages in polysaccharides of three or more ?-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. ?-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.

General description

?-Amylases belongs to the class of amylolytic enzymes.

We are committed to bringing you?Greener?Alternative Products, which adhere to one or more of The 12 Principles of?Greener?Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

10000, 25000, 50000, 100000 units in glass bottle

Physical form

Crystalline suspension in 2.3 M (NH₄)₂SO₄

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH?4.8 at 20?°C.