Acrylamide/Bis-acrylamide, BioReagent, for molecular biology, 49:1 (ratio)

Stock Code: 3586549
Manufacturer Part No: A0924-1L

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Descriptions

Application

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

Biochem/physiol Actions

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Packaging

1 L in poly bottle

100 mL in poly bottle

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution. For the 1 Liter size, use 630 mL of water.

Specifications

Quality Level	200
InChI key	OCQZXMCGTAWGEQ-UHFFFAOYSA-N
InChI	1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
Manufacturer	SIGMA-ALDRICH
Product Line	BioReagent
Form	powder