Acridine Orange hemi(zinc chloride) salt, For nucleic acid staining in cells or gels

Acridine Orange hemi(zinc chloride) salt, For nucleic acid staining in cells or gels

Stock Code: 3581798
Manufacturer Part No: A6014-100G
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£686.68 (exc VAT) per 100G
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Application


Suitable for detection of nucleic acids separated by gel electrophoresis fluorescence and epifluorescence microscopy analysis of mitochondria and lysosomes by flow cytometry DNA staining in apoptosis studies


Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.


Features and Benefits


120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels differential staining of single- and double-stranded polynucleotides


General description


Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.


Packaging


10, 25, 100 g in glass bottle


Principle


Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.

Quality Level200
InChI keyRAHGLSRJKRXOSY-UHFFFAOYSA-L
InChI1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
ManufacturerSIGMA-ALDRICH
CompositionDye content, ~80%
Product LineBioReagent
Solubility4 mg/mL (2-methoxyethanol (EGME)), ethanol: 2 mg/mL, water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
Formpowder

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