Aconitase from porcine heart,

Stock Code: 3578982
Manufacturer Part No: A5384-250MG
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£890.04 (exc VAT) per 250MG
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Analysis Note


Crude preparation containing aconitase activity


Application


Aconitase may be usefull in diabetes research as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases .


Biochem/physiol Actions


Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.


Packaging


150, 250 mg in serum bottle


Unit Definition


One unit will convert 1.0 µmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formsolid

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