BCIP/NBT solution, premixed has been used:as a substrate to develop dot-blotsas a substrate to reveal alkaline phosphatase in the immunohistochemical analysisto detect specific immune complexes in western blot analysis
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes
0.48?mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2
Substrates
Substrate for visualizing alkaline phosphatase conjugates in Western blotting.