Calpain Activity Assay Kit; Fluorogenic

Stock Code: 3587301
Manufacturer Part No: QIA120-1KIT

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Descriptions

Analysis Note

Positive ControlCalpain 1

Components

Calibration Standard, Positive Control Calpain 1, Substrate, Activation Buffer, Inhibition Buffer, Reduction Agent, Assay Buffer, Cell Lysis Buffer, 96-Well Plate, Plate Sealer, and a user protocol.

General description

Calpains are Ca²⁺-dependent proteinases, which are involved in normal cellular processes including cell proliferation, apoptosis, differentiation, and cell migration. Requires a fluorimeter or microplate reader capable of measuring fluorescence at wavelengths of Excitation max: ~360 nm and Emission max: ~460 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Dainese, E., et al. 2002. J. Biol. Chem.277, 40296.Glading, A., et al. 2000. J. Biol. Chem.275, 23908.Ishikara, I., et al. 2000. Neurosci. Lett.279, 97.Nakagawa, T. and Yuan, J. 2000. J. Cell Biol.150, 887.Pariat, M., et al. 2000. Biochem. J.345, 129.Pink, J.J., et al. 2000.Exp. Cell Res.255, 144.Wang, K.K. 2000. Trends Neurosci.23, 20.Reddy, R.K., et al. 1999. J. Biol. Chem.274, 28476.Leist, M., et al. 1998. Mol. Pharmacol.54, 789.Melloni, E., et al. 1998. J. Biol. Chem.273, 12827.Villa, P.G., et al. 1998. J. Cell Sci.111, 713.Wood, D.E., et al. 1998. Oncogene17, 1069.Kubbutat, M.H. and Vousden, K.H. 1997. Mol. Cell Biol.17, 460.Molinari, M. and Carafoli, E. 1997. J. Membr. Biol.156, 1.Sorimachi, H., et al. 1997. Biochem. J.328, 721.Squier, M.K. and Cohen, J.J. 1997. J. Immunol.158, 3690.Aoki, K., et al. 1986. FEBS Lett.205, 313.Ohno, S., et al. 1986. Nucleic Acid Res.14, 5559.

Packaging

1 kit in Glass bottle

Preparation Note

1. Cell lysates: a. Wash cell pellet with ice-cold PBS.b. Add 500-1000 µl Lysis Buffer (approximately 1 ml per 1 x 10⁷ cells) and incubate on ice for 30 min.c. Vortex and centrifuge the lysate at 14,000 x g in a pre-cooled tabletop microcentrifuge.d. Transfer the supernatant to a fresh tube immediately and discard the pellet.e. Dilute the lysate at least 1:10 before determining the protein concentration using a BCA protein assay.2. Dilute samples with Assay Buffer if necessary. Typically, cell lysates with a protein concentration >3 mg/ml should be diluted 5-fold. If the measured RFU exceeds 6000 the sample should be diluted further.

Note: All reagents necessary to perform the assay are supplied with this kit