Bicinchoninic Acid Kit for Protein Determination, for 200-1000 mug/ml protein

Stock Code: 3586659
Manufacturer Part No: BCA1-1KT
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£612.86 (exc VAT) per 1KIT
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Application


Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.


Features and Benefits


Simple, sensitive colorimetric assay for proteins Two stable reagents are used for the working solution Faster and easier than the Lowry protein assay Compatible with many detergents both ionic and nonionic Less variation between different proteins than the Bradford dye-binding assay Adaptable for use in microwell plates


Principle


Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C

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