CHROMATIN IP (CHIP) ASSAY KIT 1KIT

CHROMATIN IP (CHIP) ASSAY KIT 1KIT

Stock Code: 3586419
Manufacturer Part No: 17-295
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£654.64 (exc VAT) per EACH
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Components


Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)All necessary buffers


General description


For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.


Packaging


1 kit in Kit capacity: 22 Assays


Kit capacity: 22 Assays


Physical form


Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).


Quality


Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)immunoprecipitation (IP): suitable

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