Trypsin inhibitor from Glycine max (soybean), Isoelectric focusing marker, pI 4.6

Trypsin inhibitor from Glycine max (soybean), Isoelectric focusing marker, pI 4.6

Stock Code: 3586234
Manufacturer Part No: T1021-1VL
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Biochem/physiol Actions


This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.


Components


The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.


Preparation Note


The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.


Unit Definition


One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilityconcentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, serum-free medium: soluble, balanced salt solution: 1 mg/mL, water: 10 mg/mL
Formpowder

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