SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagge

SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagge

Stock Code: 3586233
Manufacturer Part No: S8830-20TAB
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Application


The broad protection afforded allows SIGMAFAST™ Protease Inhibitor Cocktail Tablets to be used for the purification of histidine-tagged proteins, as a general-purpose protease inhibitor cocktail and as components of buffers for cell lysis. The tablets have also been optimized and tested for use with HIS-Select media.


Components


AEBSFBestatinE-64Pepstatin APhosphoramidonLeupeptinAprotinin


General description


SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free is a tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metalloproteases. The tablets have been formulated to be compatible with sample processing by immobilized metal affinity chromatography (IMAC) such as used for the purification of histidine-tagged proteins. The tablets contain no strong metal chelators, such as EDTA, which might interfere with IMAC processing. Finally, the tablets contain a unique, proprietary formulation including a pepstatin A salt to provide additional protection not normally obtained in a tablet format.


Legal Information


SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC


Physical form


Tablet that is water soluble


Quantity


One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.


Specificity


Inhibits serine, cysteine, aspartic, and metalloproteases.


Storage and Stability


The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formtablet

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