1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:feline teeth mice brain and liver tissues human ectocervical epithelial cellsplacental sampleshuman mesenchymal stromal cells (hMSCs)
Features and Benefits
less toxic alternative to chloroform compatible with TRI reagent for isolation of RNA with decreased DNA contamination
General description
BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.
Other Notes
BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.