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General description
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF™ detection can be used.DNA or RNA probes are labeled during a 30 min reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 min from completion of hybridization to application of detection reagent.Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Legal Information
ECF is a trademark of Cytiva
AlkPhos DirectThis product is protected by patent numbers US 5,939,257 and EP 770678 owned by Cytiva Bio-Sciences Corp. and equivalent patents and patent applications in other countries.