AUTOSEQ G-50

AUTOSEQ G-50

Stock Code: 2794882
Manufacturer Part No: GE27-5340-01
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£214.50 (exc VAT) per EACH
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Analysis Note


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Application


illustra™ Autoseq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data.llustra Autoseq™ G-50 consists of MicroSpin™ columns containing Sephadex® G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.illustra™ Autoseq™ G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.


Features and Benefits


For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing. Columns are prepacked with Sephadex® G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative. Columns that are ready to use enable sample application to collection of purified product in less than 4 min.


General description


illustra™ Autoseq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results.


Legal Information


AutoSeq is a trademark of Cytiva


MicroSpin is a trademark of Cytiva


illustra is a trademark of Cytiva


Sephadex is a registered trademark of Cytiva

Particle size50-150 µm (dry)

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